Coding
Part:BBa_K1199045
Designed by: Tian Wang Group: iGEM13_UESTC_Life (2013-09-17)
LinA-F2A-LinB F2A can cleave LinA and LinB to mediate gamma-HCH
F2A peptide sequence is in foot and mouth diease virus gene.it link LinA and LinB gene and in the end of LinA gene don't have stop coden. When it is expressd in E.coli, LinA and LinB are cleaved by F2A peptide. Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 532
Illegal XhoI site found at 469 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 694
Illegal NgoMIV site found at 1185
Illegal NgoMIV site found at 1219
Illegal AgeI site found at 619
Illegal AgeI site found at 1285 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
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